PURPOSE: The aim of this study is to evaluate which cryopreservation protocol, freezing before or after swim-up, optimizes cryopreservation outcomes in terms of motile sperm count, motility, morphology, and viability, and also to establish whether sperm viability could be assessed based on sperm motility. In parallel, total motility evaluation by CASA system computer-assisted sperm analyzer and hypoosmotic swelling test HOS-test was performed in each sample to compare the viability results of both methods. In the second experiment, 21 normozoospermic semen samples and 20 semen samples from male factor patients were included. After fresh ejaculate evaluation, the semen sample of each patient was divided into two aliquots, one of them was frozen before swim-up and the other was frozen after swim-up. Motility, sperm count, morphology, and viability were evaluated after thawing. In fact, sperm selection prior to freezing reaches critical values when subfertile patients are considered. In addition, sperm freezing prior to swim-up selection must be considered in order to achieve better outcomes after thawing, especially in patients presenting poor sperm baseline.
Sperm freezing | Human Fertilisation and Embryology Authority
Sperm freezing, or cryopreservation, is the process of preserving sperm cells for later use, which was introduced in the s. Cryopreserved specimens, when properly stored, can remain viable indefinitely. Pregnancies have been reported in cases where the sperm was stored for 20 years before thawing and insemination. The man may be given a choice of producing a specimen at home or at the lab site. Men who opt to give a specimen at the lab will be given a private room and visual material.
Semina: Ciências Agrárias
User Username Password Remember me. Font Size. Journal Help. Open Journal Systems. Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates.
Effect of freezing and thawing rates on sperm motility in Bocachico Prochilodus magdalenae Pisces, Characiformes. To determine the freezing and thawing rates necessary to maintain sperm viability during cryopreservation of Bocachico semen. Materials and methods.